![]() ![]() In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.Īctin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. View All beta-actin loading contorl antibodies. OriGene offers high quality quality of Anti-beta actin mouse mAb (Loading control) for Western Blot. In contrast, filament nucleation by the Arp2/3 complex is not affected. Beta-actin, is usually used as a loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. Actin, cytoplasmic 1, N-terminally processed: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. Western blotting analysis is the most common method for identification and quantification of a specific protein in complex protein mixtures (24). Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICA元) to form methionine sulfoxide promotes actin filament depolymerization. Anti-actin antibody, which associates with both G- and F-actin, in conjunction with fluorescent secondary antibody produced a pattern similar to that obtained by simultaneous visualization with fluorescein-DNAse I and BODIPY 581/591- or rhodamine-phalloidin. ![]()
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